Charles alienated two powerful factions in Scottish society through his actions. Firstly, there were the Presbyterians, who believed that Christ, not the King, was the head of the Kirk, and that spiritual power should flow from the Kirk Elders upwards and not from the King down. In his attempts to tamper with religion, Charles gave the Presbyterians political credibility. Secondly, by introducing bishops into government, Charles had weakened the traditional role of the Scots nobility. Disaffected, they drew closer to the Presbyterian radicals. The crunch came in 1637 when Charles insisted, without consultation, on introducing an English-style prayerbook into Scotland. It incited a revolution - the National Covenant was signed at Greyfriars Kirk, Edinburgh, in 1638.
The signing of the National Covenant has been called the biggest event in Scottish history. In essence it was a document, a contract with God, signed by the Nobles, Ministers and thousands of ordinary Scots, who pledged themselves to defend Scotlands rights by stating what they would and wouldn't agree to in matters of Kirk and state. Drawn up by two of Scotlands sharpest minds, Archibald Johnston of Wariston and Alexander Henderson, it contained radical demands for changes in Scotland's governance.
The Covenant demanded a free Scottish Parliament and a free General Assembly, which means free from the Kings interference. Specifically, it demanded the abolition of bishops, who had blindly served the King in matters of Kirk and State, and, in effect, it limited the power of the King by inflating the role of Scotlands nobles and Kirk. The medieval order of divinely appointed Kings was truely over.
http://www.bbc.co.uk/history/scottishhistory/union/features_union_covenanters.shtml